Increase of Intracellular free [CA2+] in Single Human Motile Spermatozoa Treated with Human Follicular Fluid
نویسندگان
چکیده
منابع مشابه
Chromosome aberrations in human spermatozoa treated with Ca2+ ionophore A23187.
Incorporation of A23187 ionophore into the human-hamster fertilization system clearly improves the ability of human spermatozoa to penetrate zona-free hamster oocytes. Thus, an increasing number of laboratories working in human sperm cytogenetics have substituted classical incubation with Biggers-Whitten-Whittingham (BWW) medium plus human serum albumin (HSA) by pretreatment of spermatozoa with...
متن کاملComplex CatSper-dependent and independent [Ca2+]i signalling in human spermatozoa induced by follicular fluid
STUDY QUESTION Does progesterone in human follicular fluid (hFF) activate CatSper and do other components of hFF modulate this effect and/or contribute separately to hFF-induced Ca2+ signaling? SUMMARY ANSWER hFF potently stimulates CatSper and increases [Ca2+]i, primarily due to high concentrations of progesterone, however, other components of hFF also contribute to [Ca2+]i signaling, includ...
متن کاملEncoding of progesterone stimulus intensity by intracellular [Ca2+] ([Ca2+]i) in human spermatozoa.
Progesterone induces a biphasic Ca(2+) influx and consequent acrosome reaction in human spermatozoa. We have used two procedures to vary the stimulus (dosage and prior receptor desensitization) to investigate the encoding of stimulus strength by intracellular [Ca(2+)] ([Ca(2+)](i)). Acrosome reaction and amplitude (but not kinetics) of the transient [Ca(2+)](i) response (population measurement)...
متن کاملIntracellular free Ca2+ concentration in fowl spermatozoa and its relationship to motility and respiration in spermatozoa.
The ability of fowl spermatozoa to accumulate and de-esterify the intracellular fluorescent Ca2+ indicator fura-2 was established. The cytosolic Ca2+ concentrations, measured by this technique, did not change after the addition of 1 mmol EGTA l-1. Subsequently, addition of the calcium ionophore A23187 caused a reduction in cytosolic Ca2+ concentrations, presumably by efflux of Ca2+ from the spe...
متن کاملThe Effect of Human Follicular Fluid (FF) on Preimplantation Mouse Embryo Development in Vitro
SUMMARY Since at oocyte retrieval for in vitro fertilization there is some FF become incorporate to egg, we decided to investigate the effect of this fluid on preimplantation embryo development in vitro. FF was obtained at the time of oocyte retrieval for IVF, centrifuged and then either inactivated by heat and stored at 4°c or without inactivation stored at-20°c until used. Late 2-cell emb...
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ژورنال
عنوان ژورنال: Archives of Andrology
سال: 1995
ISSN: 0148-5016
DOI: 10.3109/01485019508987839